Describe the Use of Restriction Enzymes in Genetic Engineering
They are used in RFLP techniques to cut the DNA into smaller fragments to study the fragment length differences among the individuals. Restriction enzymes are used to cut the chromosomes into small segments which are then cut into even smaller pieces until the sought-after gene is found. Genetic Engineering Restriction Enzymes And Plasmids Recombinant Dna Technology Biofoods An extremely important use of. . Blunt end cutters Type II restriction enzymes of this class cut the DNA strands at same points on both the strands of DNA within the recognition sequence. They are specific endonuclease enzymes in the cells which first recognize the specific sequence called restriction sites within the DNA strand and cleave the phosphodiester backbone of the DNA at specific sites. Restriction enzymes are also frequently used to verify the identity of a specific DNA fragment based on the known restriction enzyme sites sequence that it contains. ...